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primary cell lines of hpasmcs  (Procell Inc)

 
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    Procell Inc primary cell lines of hpasmcs
    Primary Cell Lines Of Hpasmcs, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary cell lines of hpasmcs/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    primary cell lines of hpasmcs - by Bioz Stars, 2026-04
    90/100 stars

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    Micro-RNA-1 (miR-1) overexpression inhibits human pulmonary artery smooth muscle cell <t>(hPASMC)</t> <t>proliferation</t> in normoxia and hypoxia. Overexpression of miR-1 mimics in hPASMCs (50–100 nmol/l) significantly reduces proliferation in normoxia (A) and hypoxia (3% O2, 24–48 h; B). C and D: representative images of hPASMCs demonstrating miR-1 mimic overexpression in normoxia and hypoxia reduces the rate of proliferation (24 h, ×10 magnification). Representative staining images (E) and quantification of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (F) demonstrating induction of apoptosis at low levels by overexpression of miR-1 mimics (100 nmol/l) in hPASMCs (24 h, ×10 magnification). Data are represented as percentage of TUNEL-positive cells of total 4′,6-diamidino-2-phenylindole (DAPI)-positive cells per high-power field per condition (≥5 fields counted). Here, n ≥ 5 per condition. **P < 0.01, ***P < 0.001 relative to control (Ctrl). BrdU, 5-bromo-2′-deoxyuridine.
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    primary hpasmc cell line  (Lonza)
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    Lonza primary hpasmc cell line
    Micro-RNA-1 (miR-1) overexpression inhibits human pulmonary artery smooth muscle cell <t>(hPASMC)</t> <t>proliferation</t> in normoxia and hypoxia. Overexpression of miR-1 mimics in hPASMCs (50–100 nmol/l) significantly reduces proliferation in normoxia (A) and hypoxia (3% O2, 24–48 h; B). C and D: representative images of hPASMCs demonstrating miR-1 mimic overexpression in normoxia and hypoxia reduces the rate of proliferation (24 h, ×10 magnification). Representative staining images (E) and quantification of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (F) demonstrating induction of apoptosis at low levels by overexpression of miR-1 mimics (100 nmol/l) in hPASMCs (24 h, ×10 magnification). Data are represented as percentage of TUNEL-positive cells of total 4′,6-diamidino-2-phenylindole (DAPI)-positive cells per high-power field per condition (≥5 fields counted). Here, n ≥ 5 per condition. **P < 0.01, ***P < 0.001 relative to control (Ctrl). BrdU, 5-bromo-2′-deoxyuridine.
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    Image Search Results


    Micro-RNA-1 (miR-1) overexpression inhibits human pulmonary artery smooth muscle cell (hPASMC) proliferation in normoxia and hypoxia. Overexpression of miR-1 mimics in hPASMCs (50–100 nmol/l) significantly reduces proliferation in normoxia (A) and hypoxia (3% O2, 24–48 h; B). C and D: representative images of hPASMCs demonstrating miR-1 mimic overexpression in normoxia and hypoxia reduces the rate of proliferation (24 h, ×10 magnification). Representative staining images (E) and quantification of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (F) demonstrating induction of apoptosis at low levels by overexpression of miR-1 mimics (100 nmol/l) in hPASMCs (24 h, ×10 magnification). Data are represented as percentage of TUNEL-positive cells of total 4′,6-diamidino-2-phenylindole (DAPI)-positive cells per high-power field per condition (≥5 fields counted). Here, n ≥ 5 per condition. **P < 0.01, ***P < 0.001 relative to control (Ctrl). BrdU, 5-bromo-2′-deoxyuridine.

    Journal: American Journal of Physiology - Lung Cellular and Molecular Physiology

    Article Title: Micro-RNA-1 is decreased by hypoxia and contributes to the development of pulmonary vascular remodeling via regulation of sphingosine kinase 1

    doi: 10.1152/ajplung.00057.2017

    Figure Lengend Snippet: Micro-RNA-1 (miR-1) overexpression inhibits human pulmonary artery smooth muscle cell (hPASMC) proliferation in normoxia and hypoxia. Overexpression of miR-1 mimics in hPASMCs (50–100 nmol/l) significantly reduces proliferation in normoxia (A) and hypoxia (3% O2, 24–48 h; B). C and D: representative images of hPASMCs demonstrating miR-1 mimic overexpression in normoxia and hypoxia reduces the rate of proliferation (24 h, ×10 magnification). Representative staining images (E) and quantification of terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) assay (F) demonstrating induction of apoptosis at low levels by overexpression of miR-1 mimics (100 nmol/l) in hPASMCs (24 h, ×10 magnification). Data are represented as percentage of TUNEL-positive cells of total 4′,6-diamidino-2-phenylindole (DAPI)-positive cells per high-power field per condition (≥5 fields counted). Here, n ≥ 5 per condition. **P < 0.01, ***P < 0.001 relative to control (Ctrl). BrdU, 5-bromo-2′-deoxyuridine.

    Article Snippet: In addition to these cell lines, a male primary hPASMC cell line from Lonza (CC-2581) was used for cell transfection, migration, and proliferation assays at passages 4–8 .

    Techniques: Over Expression, Staining, TUNEL Assay, Control